Test the inhibition squeeze water of flesh fruit pineapple on the growth of streptococcus mutans


  • Zalila Oktariani Politeknik Kemenkes Surabaya
  • Sri Hidayati Politeknik Kemenkes Surabaya
  • Imam Sarwo Edi Politeknik Kemenkes Surabaya
  • I.G.A Kusuma Astuti Politeknik Kemenkes Surabaya


inhibition test, squeeze water of flesh fruit pineapple


Background: Dental and oral health problems are still a major problem for Indonesian
people. The most common dental and oral disease is dental caries. Currently, it is found
that dental caries is an infectious disease with multifactorial causes. Streptococcus mutans
is the main cause of dental caries. One of the efforts that can be used to reduce dental
caries is antibacterial which can kill Streptococcus mutans bacteria. One of the natural
antibacterials that can be utilized and obtained by the community is pineapple (Ananas
comosus). The health benefits of pineapple are related to the bromelain content in
pineapple (Ananas Comosus) because it has anti-inflammatory activity, fibrinolytic
activity, and can prevent platelet aggregation.
Objective: The purpose of this study was to determine the inhibition squeeze water of
flesh fruit pineapple the growth of Streptococcus mutans bacteria.
Method: This research is an experimental laboratory research conducted from July to
August 2022 at the Microbiology Research Center Laboratory, Faculty of Dentistry,
Airlangga University, Surabaya, which is located at Jl. Mayor General. Prof. Dr. Moestopo
47 Surabaya 60132. The method in this study was to observe and measure the diameter of
the zone of inhibition of Streptococcus Mutans bacteria using the paper disc diffusion
method. From the calculated data, the Shapiro-Wilk normality test was carried out, because
based on the number of samples less than 50 samples. After the data were normally
distributed, bivariate analysis was carried out using the One Way Anova test.
Result: based on the results of statistical tests using the One-way Anova test, p-value =
0.000 was obtained so that p-value <0.05, which means that the concentration squeeze
water of flesh fruit pineapple tested has an effect on the growth of Streptococcus mutans
bacteria (p-value <0.05 ) showed that squeeze water of flesh fruit pineapple concentrations
of 25%, 50%, 75% and 100% were effective in inhibiting Streptococcus mutans bacteria.
Conclusion: Based on the results of the research that has been done, it can be it was
concluded that there were differences in the inhibition squeeze water of flesh fruit
pineapple at concentrations of 25%, 50%, 75%, and 100%.